dextran hydrodynamic radius30 Ago dextran hydrodynamic radius
7 8 (2006). Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. Materiaux 1-5. Stenekes, R.J.H. The genus Weissella: taxonomy, ecology and biotechnological potential. The increased TEWL values which were proportional to the number of microspores indicate SC disruption and formation of microchannels in the skin. The human and pig skin permeability of FITC-dextran (M.W. official website and that any information you provide is encrypted 2009;301(8):60913. Karmarkar, S.; Garber, R.: Kluza, J.; Koberda, M. (2006). In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). Ann Surg Oncol. Biophysical Journal, 87, 4259-4270. Google Scholar. Unable to load your collection due to an error, Unable to load your delegates due to an error. The hydrodynamic radius of 2-NBDG glucose was too small to measure. Soc., 21, 1, 78-86. Masuelli, Martin Alberto (2013). Bookshelf For Research Use Only. Monolayers and Thin Films of Dextran Hydrophobically Modified. This conjecture found to be correct. Chen Rong Huei; Min Lang Tsai (1998). The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. (2003). Dynamics of a Polymer Solution in a Rigid Matriz. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. 2005;304(1):27486. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which 2009;8(2):1739. J. Phys. Exp Cell Res. European Biophysical Journal, 36, 85-94. Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection Optical properties of dextran in solution and in films. The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). The solution was measured using an Anton Paar DMA35N densitometer. Progress in Polymer Science, 30, 38-70. where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. ; Aberle, T; Burchard, W. (2000). Surface morphology study of dextran by scanning electron microscopy showed the porous web like structure. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. J Cosmet Laser Ther. Peptide and protein transdermal drug delivery. WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich On the molecular weight distribution of dextran T-500. European Polymer Journal, 37, 619-622. Influence of molecular weight on Dextran The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. Hydrodynamic ; Wood, P.J. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. After 8h, the micropore became more distorted from the previous circle, suggesting that the micropores were closing. Hydrodynamic Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on. The receptor chamber was filled with PBS. Journal of Applied Polymer Science, 27, 795-810. Corp. (Seoul, Korea). In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. (1937). ; Fran, H.P. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. Expert Opin Drug Deliv. (2008). Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Harding, Stephen E. (2005). In addition, the permeation rate was higher in pig skin than in human skin. (1999). Polysaccharides-based nanoparticles as drug delivery systems. Springer Editions. The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Sachdeva V, Banga AK. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. Trop J Pharm Res. Kirkland, J. J.; Dilks, C. H.; Rementer, S. W. (1992). The Mark-Houwink exponent bears the signature of a three-dimensional configuration of a polymer chain in the solvent environment. Study of temperature influence. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. Carbohydrate Polymers 72, 356-360. In the case of the work performed in references [34, 35, 36] they studied using the number average molecular weight, which rearranges the value of a and forces it to enter into unbranched or linear molecules, on the other hand, if molecular weights are used the value would be less than 0.5. Expert Rev Med Devices. Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Thomas Gutberlet (Lipoteichoic Acid): Statistiques d'influence In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). Hydrodynamic Properties of Tragacanthin. Kasaai, Mohammad R. (2012). Radiofrequency ablation of malignant liver tumors. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Viscotek GmbH, Europe. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. Ioan, C.E. With FITC-dextran 20 K, cumulative Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. 2011;16(2):518. Provided by the Springer Nature SharedIt content-sharing initiative. Carbohydrate Polymers 99, 199-207. 1984;17(6):11703. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. Carbohydrate Polymers 88 1440-1444. Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. TEWL values before and immediately after microporation on human cadaver skin according to the number of times microproration was done (n=3). These results could be attributed to the differences in the in vivo and in vitro skin nutrients supply; with in vivo studies, the skin tissue is supplied nutrients directly from the blood vessels, but the human cadaver skin is supplied from the media, therefore, the skin tissue regeneration efficiency is low [26,27,28]. This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules. Molecular weight, molecular weight distribution and molecular size of a native dextran. Modelling the endothelial blood-CNS barriers: a method for the Molecular weight (M v) and hydrodynamic properties can be calculated from there. The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. For a values from 0-0.5 a rigid sphere is predicted in a ideal solvent, from 0.5-0.8 a random coil in good solvent, and from 0.8-2 a rigid or rod like shape is expected (stiff chain). Huggins, Maurice L. (1942). Google Scholar. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. 2013, Article ID 360239, pp. For a limited temperature range, the viscosity of a polymer solution varies generally following a relation similar to that of usual liquids (rrhenius plot), where the polymer concentration is not very high and that temperature remains far enough from glass transition [38], and for system PEAorganic solvents see reference [63]. Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. One day before the experiment, the skin was stabilized in the skin culture medium. 2004;25(9):155967. dextran A solvent at this temperature is called a theta solvent [53]. WebFITC Products. Kumar SV, Tarun P, Kumar TA. SWC designed and coordinated the research. Ubbelohde, Leo. Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. International Journal of Biological Macromolecules, 42, 133-137. Microneedles and their applications. An undergraduate physical chemistry experiment. Carbohydrate Polymers, 39, 377-380. Biomater Res 24, 22 (2020). In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. Masuelli, M. A. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. Careers. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). WebR H Hydrodynamic radius [m] The basic DLS setup. statement and Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria. The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth Correspondence to Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. 4K, b M.W. dextran (1999). Drug Deliv. (2003). The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). 2023 BioMed Central Ltd unless otherwise stated. Terms and Conditions, This site needs JavaScript to work properly. (1998). Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. ; Veldhuis, T.F.J. Macromolecules. Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. Carbohydrate Polymers, 74, 372-378. Walkenhorst, S.; Olivier, R. (2003). Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. Environ Toxicol Pharmacol. ; Weisz, J. This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. Solution properties of branched dextran. 2010;4(1):117. ; Rjumtsev, E.I. Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. In vivo iontophoretic delivery of salmon calcitonin across microporated skin. GYA primarily conducted research. Pure Appl. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. There is a deviation from linearity after 580kDa [69, 70]. Structure Properties of Dextran. Curley SA. The author thanks Universidad Nacional de San Luis (Project 2-81/11), FONCyT (PICT 2004-N23-2548 and PICT 2008-N21-84), and CONICET (PIP 6324 Res. Food Funct. Global hydrodynamic analysis of the molecular flexibility of galactomannans. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. Carbohydrate Polymers, 68, 477-488. It is well tolerated on parenteral infusion by most people but a very low incidence It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50]. pp13-21. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. GPC Symp. Polymers | Free Full-Text | Confinement-Induced Fractionation and The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. Pharm Res. Nat Biotechnol. Using the polymer standards, a plot of the ln [] vs ln Mw usually gives a straight line. The micropore formed at the SC through to the epidermis, enabling drug delivery. Carbohydrate Polymers 87, 405-410.
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